粳稻“龙锦1号/香软米1578” F3家系群糙米矿质元素含量变异及相关性分析

 利用粳稻品种间杂交组合“龙锦1号/香软米1578”的196份F3家系,对糙米中Fe、Se、Zn、Cu、Mn、Ca、Mg、K、Na和P等10种矿质元素含量的变异及其相关性进行了分析。 10种矿质元素在F3家系间均有较大的变异,其中Na含量变异最大,Zn含量变异最小,变异系数分别为77.69％和12.04％。各矿质元素含量的变异系数大小顺序为Na>Se>Cu>Fe>Mg>Mn>Ca>P>K>Zn。不同矿质元素含量也有较大的差异,F3家系群各矿质元素含量平均值高低排序为P>K>Mg>Ca>Fe>Mn>Zn>Na>Cu>Se。糙米中10种矿质元素含量在F3家系群中均表现为正态分布,为由多基因控制的数量性状。Zn与Fe、Cu,Mn与Mg、Ca、K、P,Ca与Mg、K、Na、P,Mg与K、P,P与K、Na含量呈显著或极显著正相关,而Fe与Se、Mn与Na、Mg与Na含量呈显著或极显著负相关。Mn、Ca、Mg、K、P含量与其他矿质元素含量间的相关关系较Fe、Se、Cu、Zn含量与其他矿质元素含量间的相关关系更为密切。     

Contrast-enhanced ultrasonography to assess blood perfusion of skeletal muscles in normal dogs

This study evaluated perfusion of skeletal muscle using contrast enhanced ultrasonography in humerus, radius, femur and tibia in normal dogs. Contrast enhanced ultrasonography for each region was performed after injecting 0.5 mL and 1 mL of contrast medium (SonoVue) in every dog. Blood perfusion was assessed quantitatively by measuring the peak intensity, time to the peak intensity and area under the curve from the time–intensity curve. Vascularization in skeletal muscle was qualitatively graded with a score of 0–3 according to the number of vascular signals. A parabolic shape of time–intensity curve was observed from muscles in normal dogs, and time to the peak intensity, the peak intensity and area under the curve of each muscle were not significantly different according to the appendicular regions examined and the dosage of contrast agent administered. This study reports that feasibility of contrast enhanced ultrasonography for assessment of the muscular perfusion in canine appendicular regions.     

Diffuse idiopathic skeletal hyperostosis with prominent appendicular bone proliferation in a dog

This report described radiographic and CT features of atypical diffuse idiopathic skeletal hyperostosis in a 5 year-old, female Shih-tzu showing marked proliferative bone lesions in the appendicular skeleton with minor spinal changes. Continuous or flowing bony bridge formation of vertebrae is used as the gold standard for diagnosing diffuse idiopathic skeletal hyperostosis. However, this criterion seems not to be suitable for appendicular type diffuse idiopathic skeletal hyperostosis, as in the present case. Diffuse idiopathic skeletal hyperostosis is a progressive skeletal disease, and thus, enthesophytosis and the multiple bony proliferations at insertion sites of ligaments and tendons to appendicular bones in a lamellar or trabecular pattern were considered diagnostic features of the appendicular type of diffuse idiopathic skeletal hyperostosis.     

Post-Death Cloning of Endangered Jeju Black Cattle (Korean Native Cattle): Fertility and Serum Chemistry in a Cloned Bull and Cow and Their Offspring

To preserve Jeju black cattle (JBC; endangered native Korean cattle), a pair of cattle, namely a post-death cloned JBC bull and cow, were produced by somatic cell nuclear transfer (SCNT) in a previous study. In the present study, we examined the in vitro fertilization and reproductive potentials of these post-death cloned animals. Sperm motility, in vitro fertilization and developmental capacity were examined in a post-death cloned bull (Heuk Oll Dolee) and an extinct nuclear donor bull (BK94-13). We assessed reproductive ability in another post-death cloned cow (Heuk Woo Sunee) using cloned sperm for artificial insemination (AI). There were no differences in sperm motility or developmental potential of in vitro fertilized embryos between the post-death cloned bull and its extinct nuclear donor bull; however, the embryo development ratio was slightly higher in the cloned sperm group than in the nuclear donor sperm group. After one attempt at AI, the post-death cloned JBC cow became pregnant, and gestation proceeded normally until day 287. From this post-death cloned sire and dam, a JBC male calf (Heuk Woo Dolee) was delivered naturally (weight, 25 kg). The genetic paternity/maternity of the cloned JBC bull and cow with regard to their offspring was confirmed using International Society for Animal Genetics standard microsatellite markers. Presently, Heuk Woo Dolee is 5 months of age and growing normally. In addition, there were no significant differences in blood chemistry among the post-death cloned JBC bull, the cow, their offspring and cattle bred by AI. This is the first report showing that a pair of cattle, namely, a post-death cloned JBC bull and cow, had normal fertility. Therefore, SCNT can be used effectively to increase the population of endangered JBC.     

引进亚麻荠资源的生物学及品质特性的研究

为了解国内外亚麻荠资源的产量和品质特性,筛选高产、优质的亚麻荠种质资源,以引进亚麻荠资源为试材,采用田间试验与室内品质分析相结合的方法,对引进亚麻荠资源的生物学及品质特性进行了分析。结果表明:在东经128°,北纬42°的长白山区引进亚麻荠的生育期为102~107d,籽粒产量为2 245~3 270kg·hm-2,出油率达27.00%~29.67%,粗蛋白含量为20.77%~26.17%,粗脂肪含量为25.24%~33.13%。不饱和脂肪酸油酸、亚油酸、亚麻酸含量分别高达13.70%~16.71%、12.18%~17.29%和36.39%~37.57%,3种不饱和脂肪酸总量达65.24%~67.68%。     

IMAGING DIAGNOSIS—POSITIVE CONTRAST PERITONEOGRAPHIC FEATURES OF TRUE DIAPHRAGMATIC HERNIA

A true diaphragmatic hernia is a congenital diaphragmatic malformation that can appear identical to a peritoneopericardial diaphragmatic hernia (PPDH). True diaphragmatic hernias are rare in dogs. Herein we describe the use of positive contrast peritoneography for diagnosis of a true diaphragmatic hernia in two dogs.     

利用SAGE库挖掘有功能的假设基因

利用生物信息学方法,从人大动脉内皮细胞(HAECs)低氧处理的基因表达的系列分析(SAGE)中,挖掘出许多与低氧反应相关的假设基因。共预测到369个有功能的假设基因,其表达产物大部分与信号传导和基因的表达调控相关。选择了8个候选基因,RT—PCR验证其是否真正表达,其中7个为真基因。半定量RT—PCR表明,这些基因表达水平的变化在低氧处理的人脐静脉内皮细胞(HUVECs)中得到验证。结果表明：SAGE库中包含丰富的假设基因资源,可以用生物信息学方法从中鉴定与特定生理或病理过程相关的假设基因。     

Effects of drought stress on root morphology and spatial distribution of soybean and adzuki bean

Due to global climate change, Korea is facing severe droughts that affect the planting and early vegetative periods of upland crops. Soybean and adzuki bean are important legume crops in Korea, so it is critical to understand their adaptations to water stress. This study investigated the changes in root morphological properties in soybean and adzuki bean and quantified the findings using fractal analysis. The experiment was performed at the National Institute of Crop Science in Miryang, Korea. Soybeans and adzuki beans were planted in test boxes and grown for 30 days. The boxes were filled with bed soil with various soil moisture treatments. Root images were obtained and scanned every two days, and the root properties were characterized by root length, depth and surface area, number of roots, and fractal parameters(fractal dimension and lacunarity). Root depth, length and surface area and the number of roots increased in both crops as the soil moisture content increased. The fractal dimension and lacunarity values increased as the soil moisture content increased. These results indicated that the greater the soil moisture, the more heterogeneous the root structure. Correlation analysis of the morphological properties and fractal parameters indicated that soybean and adzuki bean had different root structure developments. Both soybean and adzuki bean were sensitive to the amount of soil moisture in the early vegetative stage. Soybean required a soil moisture content greater than 70% of the field capacity to develop a full root structure, while adzuki bean required 100% of the field capacity. These results would be useful in understanding the responses of soybean and adzuki bean to water stress and managing irrigation during cultivation.     

The comparative tuberculin test in guinea pigs using PPD extracts prepared from mycobacteria killed with phenol

Summary. Purified-protein-derivative (PPD) extracts were prepared from Mycobacterium tuberculosis (PPD-S), M. bovis (PPD-BS), M. avium (PPD-A) and M. kansasii (PPD-K), after killing the cultures with phenol. The reactions were assessed in guinea pigs sensitised to a range of mycobacteria, with a view to selecting a suitable pairing of the extracts to distinguish sensitivity due to M. tuberculosis or M. bovis from that due to other mycobacteria.
Sensitisation induced by M. bovis was best distinguished from others using PPD-S and PPD-A; for sensitisation by atypical mycobacteria, PPD-BS was better than PPD-S, used with either PPD-K or PPD-A, PPD-BS was more specific than PPD-S.
Using PPD-BS, PPD-A and PPD-K in a comparative test, 3 groups were recognised: those sensitive to M. bovis or M. tuberculosis (greatest reaction was to PPD-BS); those sensitive to M. avium, M. Intracellulare and M. scrofulaceum (greatest reaction was to PPD-A); those sensitive to M. kansasii, M. marium, M. gordonae and M. fortuitum (greatest reaction was to PPD-K).     

Synthesis and herbicidal activity of new 3-(3-bromothiophen-2-yl)-5-(2,6-difluorobenzyl)oxymethyl-5-methyl-4,5-dihydroisoxazole as a paddy field herbicide

A new compound 3-(3-bromothiophen-2-yl)-5-(2,6-difluorobenzyl)oxymethyl-5-methyl-4,5-dihydroisoxazole was synthesized and the herbicidal activity was assessed under glasshouse and flooded paddy field conditions. This compound demonstrated good rice selectivity and potent herbicidal activity against annual weeds at 125 g ai ha⁻¹ under greenhouse conditions. Soil application of this compound showed the complete control of barnyardgrass to the 4th leaf stage at 250 g ai ha⁻¹. Field trials indicated that this compound, combined with pyrazosulfuron-ethyl, controlled annual and perennial weeds rapidly with good tolerance by transplanted rice seedlings from postemergence and soil application. This compound showed a low mammalian and environmental toxicity in various toxicological tests.